Basketball course optimization based on mechanical characteristics / Otimização do curso de basquete com base em características mecânicas / Optimización del curso de baloncesto en función de las características mecánicas

ABSTRACT Introduction Currently, college basketball lacks evaluation criteria, making the teaching of many classes reduced to the study of superficial movements, preventing the optimization of the sport's intrinsic mechanical characteristics. Objective Optimize the methodology of the basketball course by implementing the kinematic analysis of its key movements. Methods Four elite athletes were selected as research subjects. Infrared reflective balls were used as markers on the right elbow joint, right shoulder joint, left hip joint, right knee joint, right ankle joint, and left ankle joint, among other areas. The mechanical characteristics of the possession preparation phase, the ground takeoff phase, the takeoff stage, and the landing phase were compared, classified, and analyzed kinematically by APA software. The data were processed by SPSS software. Experimental data were classified and analyzed using independent variance, considering significant differences for P < 0.05. Results Differences in sports habits, physical conditions, and mechanical characteristics of elite athletes resulted in distinct joint angles, although within a common range. Conclusion When optimizing the basketball course, teachers should fully study the experience of professional athletes and update and optimize the basketball course for students from a more scientific and technical point of view for a better orientation in the sports teaching of the basketball course. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução Atualmente, o basquete universitário carece de critérios de avaliação, tornando o ensino de muitas classes reduzido ao estudo de movimentos superficiais, impedindo a otimização das características mecânicas intrínsecas do esporte. Objetivo Otimizar a metodologia do curso de basquete pela implementação da análise cinemática dos seus principais movimentos. Métodos Quatro atletas de elite foram selecionados como sujeitos de pesquisa. Bolas reflexivas infravermelhas foram utilizadas como marcadores na articulação do cotovelo direito, articulação do ombro direito, articulação do quadril esquerdo, articulação do joelho direito, articulação do tornozelo direito, articulação do tornozelo esquerdo entre outras áreas. As características mecânicas da fase de preparação da posse de bola, a fase de decolagem do solo, a etapa de decolagem e a fase de pouso foram comparadas, classificadas e analisadas cinematicamente pelo software APA. Os dados foram processados pelo software SPSS. Dados experimentais foram classificados e analisados por meio de variância independente, considerando diferença significativa para P < 0,05. Resultados As diferenças de hábitos esportivos, condições físicas e características mecânicas dos atletas de elite resultaram em distintos ângulos articulares, ainda que dentro de uma faixa em comum. Conclusão Ao otimizar o curso de basquete, os professores devem estudar plenamente a experiência dos atletas profissionais, atualizar e otimizar o curso de basquete aos alunos do ponto de vista mais científico e técnico, para uma melhor orientação no ensino esportivo do curso de basquete. Nível de evidência II; Estudos terapêuticos - investigação dos desfechos do tratamento.

RESUMEN Introducción En la actualidad, el baloncesto universitario carece de criterios de evaluación, lo que hace que la enseñanza de muchas clases se reduzca al estudio de movimientos superficiales, impidiendo la optimización de las características mecánicas intrínsecas del deporte. Objetivo Optimizar la metodología del curso de baloncesto mediante la aplicación del análisis cinemático de sus principales movimientos. Métodos Se seleccionaron cuatro atletas de élite como sujetos de la investigación. Se utilizaron bolas reflectantes infrarrojas como marcadores en la articulación del codo derecho, la articulación del hombro derecho, la articulación de la cadera izquierda, la articulación de la rodilla derecha, la articulación del tobillo derecho y la articulación del tobillo izquierdo, entre otras zonas. Las características mecánicas de la fase de preparación de la posesión, de la fase de despegue en tierra, de la fase de despegue y de la fase de aterrizaje fueron comparadas, clasificadas y analizadas cinemáticamente por el software APA. Los datos se procesaron con el software SPSS. Los datos experimentales fueron clasificados y analizados por varianza independiente, considerando la diferencia significativa para P < 0,05. Resultados Las diferencias en los hábitos deportivos, las condiciones físicas y las características mecánicas de los atletas de élite dieron lugar a distintos ángulos articulares, aunque dentro de un rango común. Conclusión A la hora de optimizar el curso de baloncesto, los profesores deben estudiar a fondo la experiencia de los deportistas profesionales, actualizar y optimizar el curso de baloncesto para los alumnos desde el punto de vista más científico y técnico, para una mejor orientación en la enseñanza deportiva del curso de baloncesto. Nivel de evidencia II; Estudios terapêuticos - investigación de los resultados del tratamiento.

The relationship between fine motor skills and executive function in boys with attention deficit hyperactivity disorder / 中国学校卫生

Objective@#To investigate the relationship between fine motor skills and executive function in school aged boys with attention deficit hyperactivity disorder (ADHD) aged 6-10 years, so as to provide a reference for promoting executive function.@*Methods@#From November 2022 to May 2021,65 boys with ADHD were recruited from a tertiary hospital in Beijing and a public elementary school in Beijing, and 65 boys with typical development according to age and sex were recruited as controls. Children s fine motor skills were assessed using the movement assessment battery for children, second version (MABC-2), and children s inhibitory control, working memory, and cognitive flexibility were assessed using the Stroop color word test (SCWT), Rey osterrich complex figure test (ROCFT) and trail making test (TMT), respectively. Independent samples t-tests were used to analyze the differences between the two groups, and Pearson correlation analysis and stratified regression analysis were used to explore the relationship between fine motor skills and executive functioning in boys with ADHD.@*Results@#In the fine motor skills test, boys with ADHD scored significantly lower (8.68±3.62) than control boys (11.22±2.27)( t =4.80, P <0.05); in the executive function test, the results of SCWT, ROCFT, and TMT tests were significantly worse in boys with ADHD than in normal boys ( t =-4.53-4.42, P < 0.05). Correlation analysis showed that the fine motor skill scores of boys with ADHD were negatively correlated with the number of word sense errors ( r =-0.35) and color errors ( r =-0.42), and positively correlated with memory scores for delayed structure ( r =0.30) and detail ( r =0.25), which were negatively correlated with TMT-A ( r =-0.34),TMT-B reaction time ( r =-0.26), number of errors ( r =-0.43) ( P <0.05). Stratified regression analysis showed that fine motor skill scores of boys with ADHD were predictive of test results for inhibitory control, working memory and cognitive flexibility, with explanatory rates of 15%, 7%, and 19%, respectively.@*Conclusion@#Fine motor skills in boys with ADHD are correlated with executive functions, and fine motor skills could predict inhibitory control, working memory and cognitive flexibility to some extent. The development of fine motor skills in boy with ADHD could promote their executive functions.

Acupuncture at sphenopalatine ganglion combined with conventional acupuncture for episodic cluster headache: a randomized controlled trial / 中国针灸

OBJECTIVE@#To observe the clinical effect of acupuncture at sphenopalatine ganglion combined with conventional acupuncture for episodic cluster headache (CH).@*METHODS@#One hundred and eighty patients with episodic CH were randomly divided into a combined group (60 cases, 3 cases dropped off)，an acupuncture group (60 cases, 2 cases dropped off) and a sphenopalatine ganglion group (60 cases, 2 cases dropped off and 1 case was removed). The patients in the acupuncture group were treated with conventional acupuncture at Touwei (ST 8), Yintang (GV 24+), Yangbai (GB 14), Hegu (LI 4), etc., once a day, 6 times a week. The patients in the sphenopalatine ganglion group were treated with acupuncture at sphenopalatine ganglion, once every other day, 3 times a week. On the basis of the conventional acupuncture, the combined group was treated with acupuncture at sphenopalatine ganglion once every other day. Two weeks were taken as a course of treatment, and 3 courses of treatment were required in the 3 groups. The score of visual analogue scale (VAS), the number of headache attacks per week, the duration of each headache attack and the score of migraine-specific quality of life questionnaire version 2.1 (MSQ) were observed before and after treatment and in follow-up of 3 months after treatment. The clinical efficacy of each group was compared.@*RESULTS@#After treatment and in follow-up, the VAS score of headache, the number of headache attacks per week, the duration of each headache attack, and each various scores and the total score of MSQ of each group were lower than those before treatment (P<0.01). Except that the number of headache attacks per week in the combined group was lower than the sphenopalatine ganglion group (P<0.01), other indexes in the combined group were lower than the other two groups (P<0.05, P<0.01). The total effective rate in the combined group was 93.0% (53/57), which was higher than 75.9% (44/58) in the acupuncture group and 73.7% (42/57) in the sphenopalatine ganglion group（P<0.05, P<0.01).@*CONCLUSION@#Acupuncture at sphenopalatine ganglion combined with conventional acupuncture could reduce the degree of pain in patients with episodic CH, reduce the number and duration of headache attacks, and improve the quality of life of patients. It is more effective than simple conventional acupuncture or acupuncture at sphenopalatine ganglion alone.

Family history of esophageal cancer modifies the association of serum lipids and malignant esophageal lesions: a nested case-control study from the "Endoscopic Screening for Esophageal Cancer in China" trial / 中华医学杂志(英文版)

BACKGROUND@#The association of lipids and cancer has varied greatly among different cancer types, lipid components and study populations. This study is aimed to investigate the association of serum lipids and the risk of malignant lesions in esophageal squamous epithelium.@*METHODS@#In the "Endoscopic Screening for Esophageal Cancer in China" (ESECC) trial, serum samples were collected and tested for total cholesterol (TC), triglycerides, low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol at the time of subject enrollment. Cases were defined as malignant esophageal lesions identified by baseline endoscopic examination or by follow-up to May 31, 2018. Controls were randomly selected using incidence density sampling in the same cohort. Conditional logistic models were applied to identify the association of serum lipids and the risk of malignant esophageal lesions. Effect modification was evaluated by testing interaction terms of the factor under assessment and these serum lipid indicators.@*RESULTS@#No consistent association between serum lipid levels and esophageal malignant lesions were found in a pooled analysis of 211 cases and 2101 controls. For individuals with a family history of esophageal cancer (EC), high TC, and LDL-C were associated with a significantly increased risk of having malignant lesions (odds ratio [OR]High vs. Low TC = 2.22, 95% confidence interval [CI]: 1.14-4.35; ORHigh vs. Low LDL-C = 1.93, 95% CI: 1.01-3.65). However, a negative association was observed in participants without an EC family history (ORHigh vs. Low TC = 0.69, 95% CI: 0.48-0.98, Pinteraction = 0.002; ORHigh vs. Low LDL-C = 0.50, 95% CI: 0.34-0.76, Pinteraction < 0.001).@*CONCLUSIONS@#In this study, we found that the association of serum lipids and malignant esophageal lesions might be modified by EC family history. The stratified analysis would be crucial for population-based studies investigating the association of serum lipids and cancer. The mechanism by which a family history of EC modifies this association warrants further investigation.

Effect of aflibercept on the expression of signal transducers and activators of transcription 3 in rat retinal Müller cells / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To investigate the effect of aflibercept on the expression of signal transducers and activators of transcription 3(STAT3)in rat retinal Müller cells. <p>METHODS: The rat retinal Müller cells treated with different concentrations of Aflibercept 100 μL(diluted concentrations of 400, 200, 100 pg/mL, respectively). MTT assay were used to detect cell proliferation, flow cytometry. Apoptosis were detected by the instrument, cell invasion were detected by transwell chamber method, and protein(AKT, STAT3, GAPDH)expression were detected by Western-blot method.<p>RESULTS: The proliferation activity of Müller cells were decreased with the increased of aflibercept concentration, and compared the difference were statistically significant(<i>P</i><0.05). After treatment for 48h, the apoptotic rate of Müller cells were gradually increased with the increased of aflibercept concentration, and the invasion and penetration index of Müller cells gradually were decreased, and compared the difference were statistically significant(<i>P</i><0.05). After 48 h of transfection, the relative expression of AKT protein in Müller cells were not change significantly with the increased of Aflibercept concentration(<i>P</i>>0.05), and the relative expression of STAT3 protein decreased gradually, and compared the difference were statistically significant(<i>P</i><0.05).<p>CONCLUSION: Aflibercept can inhibit the expression of STAT3 protein in rat retinal Müller cells, thereby inhibit cell proliferation and invasion and promote apoptosis.

Integrative Analysis Confirmed the Association between Osteoprotegerin and Osteoporosis / 中国医学科学杂志(英文版)

Objective This study aimed to verify the association between osteoprotegerin gene () and its variants with osteoporosis (OP) by performing integrative analysis.Methods We used the KGG software to perform gene-based association analysis, which integrated all publicly available single-nucleotide polymorphism (SNP)-based values and obtained an overall value for the . The significant SNPs were screened for expression quantitative trait loci (eQTLs). Meta-analysis was used to combine the associations between the variants of and bone mineral density (BMD) reported in the literatures. Then we performed dual-luciferase reporter gene systems for the functional verification of the variants of .Results In the gene-based association analysis, the over all value of was 6.24×10 for BMD at femoral neck (FN) and 7.37×10 for BMD at lumbar spine (LS), indicating the importance of for OP. The publicly available eQTL database identified 5 eQTLs which exert cis-regulation effects on at FN and LS. Literature searching found that rs2073617 (known as T950C) was the hot spot SNP. There were 13 relevant studies on rs2073617 besides the GEFOS-2 study identified from the PubMed. Significant differences among TT, TC and CC genotypes at FN (= 0.047) and LS (= 0.025) were shown by meta-analysis, demonstrating the associations between T950C polymorphism and BMD. Luciferase gene expression was significantly higher at the presence of allele C than allele T in the 293T cells (=-9.47, <0.01). Conclusion The integrative analysis further confirmed the importance of in OP and the correlation of T950C polymorphism with BMD of OP. The strategy can be used as a reference for functional interpretation of other disease-related genes.

Effects of Aflibercept on ion channel of retinal Müller cell membrane cultured <i>in vitro</i> / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To investigate the effects of Aflibercept on the K+ channel of retinal Müller cell membrane cultured <i>in vitro</i>.<p>METHODS: Human Müller cells were divided into 3 groups(control group, high glucose group and experimental group). The control group were cultured in conventional DMEM medium; the high glucose group were cultured in high glucose DMEM medium; the experimental group were cultured with high glucose DMEM medium and 100μmol/L Aflibercept, and the K+ concentration of the cells were detected by MQAE, and the cell survival were detected by MTT assay, the flow cytometry were used to detect apoptosis rates, Western blot analysis were used to detect the Müller cell caspase-3 protein levels.<p>RESULTS: The Müller cells were positive for glutamine synthetase(GS)after 48h of culture, and the purification degree were above 90%. The relative concentrations of K+ in the control group, high glucose group and experimental group were(2.14±0.44)%,(23.11±4.39)%,(5.20±0.92)%, and cell viability were(100.00±0.00)%, respectively(73.24±4.13)%,(85.22±5.33)%, the apoptosis rates were(5.03±1.91)%,(26.73±3.14)%,(16.63±2.73)%, and compared the differences between two groups were statistically significant(<i>P</i><0.05). Compared with the control group, the level of caspase-3 protein in the high glucose group Müller cells were increased significantly(<i>P</i><0.05); compared with the high glucose group Müller cells, the caspase-3 protein level in the experimental group Müller cells were decreased significantly(<i>P</i><0.05).<p>CONCLUSION: Aflibercept can inhibit the K+ channel of retinal Müller cells <i>in vitro</i>, inhibit the apoptosis of Müller cells induced by high glucose, decrease the expression of caspase-3 protein, and promote cell proliferation.

Warming Needle Moxibustion Relieves Symptoms of Lumbar Disc Herniation Patients and Upregulates Plasma β-endorphin / 针刺研究

OBJECTIVE: To analyze the effect of warming needle moxibustion of Jiaji (EX-B 2), Huantiao (GB 30), etc. on lumbago and other symptoms of lumbar disc herniation (LDH) patients and changes of plasma β-endorphin (β-EP) content. METHODS: A total of 60 LDH patients were equally randomized into warming needle moxibustion group and conventional acupuncture group. Patients of the conventional acupuncture group were treated by puncturing lumbar Jiaji (EX-B 2), Huantiao (GB 30), Weizhong (BL 40), and Yanglingquan (GB 34) with filiform acupuncture needles. Patients of the warming needle moxibustion group were treated by puncturing the same 4 acupoints, and with the acupuncture needle in lumbar EX-B 2 attached an ignited moxa-stick segment. The treatment in both groups lasted for 30 min every time, once every other day for 15 times, with 5 times being a therapeutic course and two days' rest between every two courses. The therapeutic effect was evaluated according to the "Criteria for Evaluating the Therapeutic Effect of Disorders of Traditional Chinese Medicine". The lumbago severity was assessed using visual analogue scale (VAS), and the lumbar functional activity assessed using modified Oswestry dysfunction (in objects-lifting, sitting, standing, walking, sleeping, pain, activity of daily living, social activity, outing, etc.) index (ODI). The plasma β-EP content was assayed using ELISA. RESULTS: After the treatment, of the two 30 cases in the conventional acupuncture and warming needle moxibustion groups, 8 (26.67%) and 12 (40.00%) were under control, 8 (26.67%) and 10 (33.33%) had a marked improvement, 3 (10.00%) and 4 (13.33%) were effective, and 11 (36.66%) and 4 (13.33%) ineffective, with the effective rates being 63.34% and 86.66%, respectively. The effective rate of the warming needle moxibustion was significantly higher than that of the conventional acupuncture (P<0.05). The VAS scores and ODI values of the two groups were gradually decreased along with the prolongation of treatment, and significantly lower in the warming needle moxibustion group than in the acupuncture group at the 1st, 2nd and 3rd course of treatment (P<0.01). The levels of plasma β-EP were gradually increased in the two groups at the 1st, 2nd and 3rd course of treatment, and were obviously higher in the warming needle moxibustion group than in the conventional acupuncture group at each of the 3 courses (P<0.01). No obvious adverse reactions were observed in the two groups. CONCLUSION: Warming needle moxibustion has a better therapeutic effect in relieving lumbago and lumbar dysfunction, and can up-regulate blood β-EP level.

Separation and Identification of Microplastics in Digestive System of Bivalves / 分析化学

As a new type of pollutants,microplastics (small pieces of plastic with the longest dimension less than 5 mm) are bringing wide concerns by policy makers and researchers. The impact of microplastics on marine life is closely related to human health. Bivalves, as filter feeding creatures, can easily ingest microplastics in their feeding process. The microplastics in these bivalves can easily enter the human body through the food chain. In this study,a bivalve sample pretreatment method was established for separating the microplastics in their digestive system. Qualitative and quantitative analysis of microplastics was carried out by micro-Fourier transformed infrared spectroscope (μ-FT-IR) and Stereo microscope. By comparing the digestion systems of using 10% KOH and 30% H2O2, respectively, it was found that the digestion system using 10% KOH had better digestion efficiency. The recoveries of polypropylene (PP),polyethylene (PE), polystyrene (PS) and polyvinyl chloride (PVC) ranged from 96.7% to 98.6%, with RSD of less than 3.2%. Therefore, 10% KOH was used to digest organic tissue for separating the microplastics from the bivalves'digestive system. With this digestion system,the microplastics in Chlamys farreri from local markets and Mytilus galloprovincialis from both local markets and wild environments in Qingdao were separated and analyzed. The results showed that microplastics were found in over 80% of the individuals purchased from the market and 40% in the collected wild individuals. The average abundance of microplastics in Chlamys farreri purchased from different markets varied from 5.2 to 19.4 items/individual and 3.2-7.1 items/g, while in Mytilus galloprovincialis, the numbers varied from 1.9 to 9.6 items/individual and 2.0-12.8 items/g. Farmed mussels contained more microplastics (average 1. 9 items/individual, 3. 17 items/g) than wild mussels (average 0.53 items/individual, 2.0 items/g). There were various colors of microplastics. The detected microplastics came from three shapes: fibers, fragments and granules. Fibrous microplastics, being the most dominant ones,accounted for 84.11%. The average size of fibrous microplastics(0.66±0.70 mm) was larger than that of the other two shapes of microplastics. The number of microplastics decreased with increasing microplastic sizes. Microplastics of less than 500 μm coming from different markets were in the range of 26% to 84%. There was great spatial difference in the size of microplastics. The polymer composition of the microplastic was identified by μ-FT-IR. And it was found that the most common polymer component sin the samples were cellophane(CP),followed by polypropylene(PP). In the farmed mussels,PP was the most abundant component. The results showed that the abundance and types of microplastics in cultured or wild bivalves were closely related to their growing environment.

The effect of gestational diabetes mellitus on the newborn metabolism related indexes and prognosis / 重庆医学

Objective Investigate the effect of gestational diabetes mellitus (GDM)on the neonatal metabolism and prognosis.Methods A total of 265 singleton pregnancy GDM patients were collected as the observation group,while 260 cases of healthy singleton pregnant women were chosen as the control group.Detected the levels of adiponectin,insulin,C-peptide,glycated hemoglobin and blood glucose,compared the prognosis of two groups.Results Compared with the control group,adiponcetin and blood glucose in 2 hours after birth in observation group were lower,insulin,C-peptide and glycated hemoglobin were higher,the number of cesarean section was more,the difference was statically significant (P＜0.05).The incidence of low birth weight infant,macrosomia,neonatal asphyxia,neonatal hypoglycemia,neonatal hyperbilirubinemia,neonatal pneumonia,fetal death and newborn teratogenesis in the observation group was higher than that in the control group,the difference was statically significant (P＜0.05).Conclusion GDM could cause metabolic disorder and affect fetal growth and development,leading to poor prognosis.

Correlation among ambulatory blood pressure indexes and P wave dispersion in patients with hyperten-sion / 心血管康复医学杂志

Objective :To explore P wave dispersion (Pd) change in patients with hypertension ,and study correlation among ambulatory blood pressure indexes and Pd .Methods : A total of 100 patients with hypertension treated in our hospital from Jul 2015 to Jul 2017 were selected as hypertension group .Another 76 healthy subjects without cardio-vascular diseases undergoing physical examination during the same period were selected as normal control group .Ac-cording to circadian rhythm of blood pressure ,hypertension group was further divided into dipper group (n＝36 ) and non-dipper group (n＝64).All subjects received 24h ambulatory blood pressure monitoring and 12-lead ECG .P wave maximum time limit (Pmax) ,P wave minimum time limit (Pmin) and Pd were compared among all groups ,and correlation among ambulatory blood pressure indexes and Pd were analyzed .Results : Compared with normal con-trol group ,there were significant rise in Pmax [ (103.13 ± 9.19) ms vs.(118.04 ± 11.14) ms] and Pd [ (24.62 ± 5.96) ms vs.(38.05 ± 7.76) ms] in hypertension group , P＝0.001 both .Compared with dipper group ,there were significant rise in Pmax[ (116.04 ± 10.17) ms vs.(121.13 ± 12.06) ms] and Pd [ (35.05 ± 8.01) ms vs.(40.05 ± 7.16) ms] in non-dipper group , P＝ 0.035 ,0.002. Pearson correlation analysis indicated that Pd was significant positively correlated with 24h mean systolic blood pressure (SBP) ,24h SBP standard deviation (SD) ,24h diastolic blood pressure SD ,SBP load ,daytime mean SBP and nighttime mean SBP (r＝0.291～0.455 ,P＜0.05 or ＜0.01). Conclusion : SBP ,SBP load and blood pressure SD are significant positively correlated with Pd in patients with hy-pertension ,suggesting that these indexes possess higher value in assessing atrial injury in these patients .

Effects of skeletal muscle satellite cells on muscle fiber lesions / 中国组织工程研究

BACKGROUND: Skeletal muscle satellite cells have been found to possess the potential of stem cells that have the ability to self-renew with the roles of repair and regeneration. Therefore, it can be used to treat myofibrosis. OBJECTIVE: To explore the function, molecular regulation and related mechanisms of skeletal muscle satellite cells, and to promote the repair and regeneration of skeletal muscle fibers, thereby providing new ideas and intervention strategies for the rehabilitation and treatment of skeletal muscle injury. METHODS: A computer-based online search was conducted in PubMed, Mendeley, Google Scholar, and CNKI databases from 1995 to November 2016 for the relevant literature using the keywords of "skeletal muscle,satellite cells, stem cell, muscle repair". Data screening, processing, and summarization were performed. RESULTS AND CONCLUSION: According to the inclusion criteria, 51 eligible articles were enrolled. Analysis results show that skeletal muscle satellite cells are closely related to muscle fiber lesions. Based on the mechanism of skeletal muscle lesions, the regeneration and repair mechanisms of skeletal muscle satellite cells are explored. Skeletal muscle tissue is a functional and sound system that can regenerate and repair through skeletal muscle satellite cells. The function of skeletal muscle satellite cells is controlled by intracellular and extracellular information networks, which can enhance endogenous satellite cell viability. When the skeletal muscle is injured or malnourished, the viability of skeletal muscle satellite cells is inhibited, further inducing skeletal muscle fiber degeneration. Therefore, skeletal muscle fiber lesions can be treated through skeletal muscle satellite cell transplantation and stimulating the viability of endogenous skeletal muscle satellite cells, which provides intervention strategies for skeletal muscle repair and regeneration.

HPV infection of the external genitalia in men whose female partners have cervical HPV infection / 中华男科学杂志

<p><b>Objective</b>Human papilloma virus (HPV) is a necessary cause of cervical cancer and is also closely related to penile cancer, oropharyngeal cancer, and anal cancer in males. However, few studies are reported on male HPV. This study aimed to investigate HPV infection of the external genitalia in men whose female partners have cervical HPV infection.</p><p><b>METHODS</b>We collected the relevant data on the male outpatients whose partners had cervical HPV infection in our Department of Urology and Andrology from August to December 2016. We obtained samples with nylon swabs from the glans penis, corona, inner layer of the prepuce and penile body and detected different types of HPV infection using the Hybribio HPV typing kit, PCR and membrane hybridization.</p><p><b>RESULTS</b>Valid data were collected from 140 males, which showed 83.5% of HPV infection of the external genitalia, including 60 cases of HPV6 (43.2%), 27 cases of HPV16 (19.4%), 14 cases of HPV39 (10.1%), 13 cases of HPV18 (9.4%), 13 cases of HPV58 (9.4%), and 13 cases of HPV52 (9.4%). Redundant prepuce was found in 75.5% of the males, but there was no statistically significant difference in the incidence rate of HPV infection between the normal and redundant prepuce groups (P > 0.05).</p><p><b>CONCLUSIONS</b>Men who have the female partners with positive cervical HPV are at high risk of HPV infection and therefore need to be screened and treated so as to reduce HPV infection in both sexes.</p>

Hepatic Resection Combined with Radiofrequency Ablation versus Hepatic Resection Alone for Multifocal Hepatocellular Carcinomas: A Meta-analysis / 华中科技大学学报（医学）（英德文版）

This meta-analysis aimed to comprehensively assess the efficacy and safety of hepatic resection combined with radiofrequency ablation versus hepatic resection (HR) alone for the treatment of multifocal hepatoeellular carcinomas (HCC).A literature search was conducted from the database including MEDLINE,Embase,Cochrane Central Register of Controlled Trials (CENTRAL) and China Biology Medicine (CBM) disc.The primary outcomes included the 1-,3-,5-year overall survival (OS) and disease-free survival (DFS) rate.The secondary outcomes contained the intraoperative parameters and postoperative adverse events (AEs).These parameters were all analyzed by RevMan 5.3 software.After carefully screening relevant studies,four retrospective studies of high quality involving 466 patients (197 in the combined group and 269 in the HR group) were included in this study.The pooled results showed that the 1-,3-,5-year OS rate in the combined group were comparable with those in the HR group (OR=0.77,0.96,0.88;P=0.33,0.88,0.70,respectively).Similarly,there was no significant difference in 1-,3-,5-year DFS rate between the combined group and the HR alone group (OR=0.57,0.83,0.72;P=0.17,0.37,0.32,respectively).And the intraoperative parameters and postoperative AEs were also comparable between the above two cohorts.However,two included studies reported that tumor often recurred in the ablation site in the combined group.The present meta-analysis indicated that the HR combined with RFA could reach a long-term survival outcome similar to curative HR for multifocal HCC patients.And this therapy may be a promising alternative for these patients with marginal liver function or complicated tumor distribution.Furthermore,high quality randomized controlled trials (RCTs) are imperative to verify this conclusion.

Influence factors and clinical treatment of high intraocular pressure after LASEK / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To analyze the influence factors of high intraocular pressure after laser epithelial keratomileusis(LASEK), and to discuss the clinical treatment and prognosis of the high intraocular pressure patients. <p>METHODS: A retrospective study was used. From August 2013 to February 2016, 160 LASEK patients(160 eyes)in our hospital were selected. Visual acuity after operation was recorded. The incidence and influence factors of high intraocular pressure were investigated. The treatment method and prognosis of high intraocular pressure were recorded. <p>RESULTS: In the 160 patients, all patients had complete epithelial flap and different degree of photophobia. There were 19 patients with postoperative photophobia, conjunctival hyperemia and foreign body sensation significantly. At postoperative 3mo, average intraocular pressure were 18.40±4.98mmHg, which was higher than that of preoperative, 16.27±2.24mmHg(<i>P</i><0.05), and at postoperative 3mo, visual acuity was significantly higher than preoperative(<i>P</i><0.05). There were 9 patients with postoperative steroid induced ocular hypertension, the incidence rate was 5.6%; Multiple linear regression analysis showed that the cutting depth(<i>OR</i>=3.209), maximum diameter of curvature(<i>OR</i>=3.071)and fundus C/D(<i>OR</i>=9.224)value were the main risk factors leading to postoperative steroid induced ocular hypertension(<i>P</i><0.05). <p>CONCLUSION: The postoperative steroid induced ocular hypertension in LASEK is common, but has no obvious effect on visual acuity. The cutting depth, maximum diameter, curvature fundus C/D value were the main influencing factors, so we should careful use the hormone drugs in treatment and prevention. Periodic review and detection for change of intraocular pressure are needed.

Application of enteral nutrition support via naso-jejunal tube in esophageal carcinoma patients treated with ;radiotheraphy / 中国基层医药

Objective To investigate the effect of enteral nutrition support via naso-jejunal tube in esopha-geal carcinoma patients treated with radiotheraphy.Methods 36 esophageal carcinoma patients were randomly assigned into enteral nutrition(EN)group,while 38 patients assigned to control group.All patients underwent defini-tion IMRT combined with weekly concurrent chemotherapy of paclitaxel-nedaplatin.The naso -jejunal tubes were bedside inserted by hand in EN group.Enteral nutrition support began the day after the tube insertion.The control group took food orally.Nutrition was assessed through body weight,BMI,lymphocyte,albumin,pre -albumin and hemoglobin.Treatment induced complications were recorded.Results The degree of nutritional reduction was lower in EN group and significantly different with the control group.The EN group underwent (4.5 ±1 .1 )cycles concurrent chemotherapy,the control group underwent (3.1 ±2.3)cycles concurrent chemotherapy(t=6.21,P=0.027).The hematotoxicity induced by chemoradiotherapy(CRT)was statistically severe in the control group(χ2 =24.64,P<0.01),while radiation esophagitis was similar between the two groups.Conclusion EN support via naso -jejunal tube in esophageal carcinoma patients treated with radiotheraphy may improve the nutritional status,alleviate CRT induced hematotoxicity,increase tolerance of CRT.

Problems and countermeasures for communication equipment supportability of newly established field medical unit / 医疗卫生装备

Objective To explore the problems and countermeasures for the communication equipment supportability of newly established field medical unit. Methods The characteristics of the communication equipment assigned to the field medical unit were introduced, and the problems of the equipment were summarized during 2-a exercise, examination, training and etc of the field medical unit, then some countermeasures were put forward accordingly. Results The communication equipment had problems in low utilization rate, training, operation procedure and safety, and the countermeasures were brought out from the aspects of emphasis from the director, personnel training, technical performances of the communication equipment and etc. Conclusion The communication equipment needs improving to support the commanding system of the field medical unit although it behaves better than the original equipment.

Relationship of abnormal sperm DNA methylation with early spontaneous abortion / 中华男科学杂志

<p><b>Objective</b>To investigate the relationship between the abnormal sperm DNA methylation level and early spontaneous abortion.</p><p><b>METHODS</b>We randomly selected 98 males who met the inclusion criteria and whose wives suffered from unexplained abortion or embryo abortion, and included another 46 normal healthy men present for pre-pregnancy check-up as controls. We examined the semen quality and sperm morphology, obtained the sperm DNA fragmentation index (DFI) by modified sperm chromatin dispersion, and measured the sperm DNA methylation level using the methylated DNA quantification kit and the colorimetric method.</p><p><b>RESULTS</b>Compared with the normal controls, the men in the unexplained abortion group showed a significantly lower rate of big-halo sperm (［45.50 ± 26.27］ vs ［36.49 ± 23.06］%, P = 0.038), a higher rate of abnormal-head sperm (［77.08± 12.21］ vs ［81.09± 10.89］%, P = 0.049), and a lower level of sperm DNA methylation (［0.47 ± 0.33］ vs ［0.36 ± 0.26］ ng/μl, P = 0.035). The sperm DNA methylation level was positively correlated with the percentage of big-halo sperm (OR=0.546, P<0.01). Multivariate regression analysis manifested that sperm head abnormality was an independent risk factor of early spontaneous abortion or embryo abortion (OR=1.032, P = 0.049), while the high methylation level was protective factor against early spontaneous abortion or embryo abortion (OR=0.244, P = 0.03).</p><p><b>CONCLUSIONS</b>The abnormal level of sperm DNA methylation may be one of the important reasons for early spontaneous abortion or embryo abortion.</p>

Semen quality and its influencing factors in preconception males in Nanjing area / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the semen quality and its influencing factors in preconception males in Nanjing area so as to provide some evidence for working out effective intervention measures.</p><p><b>METHODS</b>Totally 687 men receiving preconceptional physical examination were enrolled in this study. A questionnaire survey was conducted among the subjects along with an analysis of their semen quality.</p><p><b>RESULTS</b>The median of sperm concentration was 63.3 x 10(6)/ml (95% CI [19.88-119] x 10(6)/ml). The median of grade a sperm was 33.03% (95% CI [19.38-55.05]%), that of grade a + b sperm was 52.08% (95% CI [39.53-69.37]%), and that of teratosperm was 91.75% (95% CI [69-100]%). The median concentration of seminal plasma PMN-elastase was 195.55 ng/ml (95% CI [76.16-3330.38] ng/ml) and that of seminal plasma zinc was 7.62 μmol/L (95% CI [1.5-23, 45] μmol/L). The positive rates of Ureaplasma urealyticum (UU), Chlamydia trachomatis (CT), and Gardnerella vaginalis (GV) were 42.4%, 0.3%, and 2.4%, respectively. The median of sperm DNA fragmentation index (DFI) of those whose wives had a history of adverse pregnancy was 20.25% (95% CI [2.15-68.25]%). Multivariate logistic regression analysis suggested that mental stress (OR 1.567, 95% CI [1.081-2.27]) and sedentariness (OR 1.772, 95% CI [1.211-2.592]) were independent risk factors for asthenospermia.</p><p><b>CONCLUSION</b>The sperm quality of preconception males in Nanjing area is not encouraging, and it can be improved by changing undesirable lifestyle and reducing mental stress.</p>

Effect of Zhuangyao Jianshen Wan (ZYJCW) on P2X1 and P2X3 mRNA expressions in rats with diuresis caused by kidney deficiency / 中国中药杂志

To investigate the urination-reducing effect and mechanism of Zhuangyao Jianshen Wan (ZYJCW). In this study, SI rats were subcutaneously injected with 150 mg · kg(-1) dose of D-galactose to prepare the sub-acute aging model and randomly divided into the model group, the Suoquan Wan group (1.17 g · kg(-1) · d(-1)), and ZYJCW high, medium and low dose groups (2.39, 1.20, 0.60 g · kg(-1) · d(-1)) , with normal rats in the blank group. They were continuously administered with drugs for eight weeks. The metabolic cage method was adopted to measure the 24 h urine volume and 5 h water load urine volume in rats. The automatic biochemistry analyzer was adopted to detect urine concentrations of Na+, Cl-, K+. The ELISA method was used to determine serum aldosterone (ALD) and antidiuretic hormone (ADH). The changes in P2X1 and P2X3 mRNA expressions in bladder tissues of rats were detected by RT-PCR. According to the results, both ZYJCW high and medium dose groups showed significant down-regulations in 24 h urine volume and 5 h water load urine volume in (P <0.05, P <0.01), declines in Na+ and Cl- concentrations in urine (P <0.01), notable rises in plasma ALD and ADH contents (P <0.05, P <0.01) and remarkable down-regulations in the P2X1 and P2X3 mRNA expressions in bladder tissues (P <0.01). The ZYJCW low dose group revealed obvious reductions in Na+ and Cl- concentrations in urine (P <0.01). The results indicated that ZYJCW may show the urination-reducing effect by down-regulating the P2X1 and P2X3 mRNA expressions in bladder tissues of rats with diuresis caused by kidney deficiency.